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arrays integrated fluidic circuits  (fluidigm)


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    Structured Review

    fluidigm arrays integrated fluidic circuits
    Arrays Integrated Fluidic Circuits, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 716 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/96+96+dynamic+array+integrated+fluidic+circuits/96%2E96+Dynamic+Array+IFC+for+Gene+Expression/pmc13039450-77-44-48
    Average 95 stars, based on 716 article reviews
    arrays integrated fluidic circuits - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: Limited effects of preterm birth and the first enteral nutrition on cerebellum morphology and gene expression in piglets
    Article Snippet: Preamplified and exonuclease‐treated cDNA was diluted 1:10 in low EDTA TE‐buffer (VWR, Bie & Berntsen, Copenhagen, Denmark) before qPCR. .. Expression analysis was performed in two 96/96 Dynamic Array Integrated Fluidic Circuits (Fluidigm, South San Francisco, CA) using TaqMan Gene Expression Master Mix (Life Technologies, Carlsbad, CA), EvaGreen 20X (VWR), and gene‐specific primers as described previously (Skovgaard et al. ). ..

    Article Title: Liver‐Directed Human Amniotic Epithelial Cell Transplantation Improves Systemic Disease Phenotype in Hurler Syndrome Mouse Model
    Article Snippet: .. Fluidigm 96.96 Dynamic Array integrated fluidic circuits were used to analyze each sample for IDUA mRNA expression and compared with that of human primary hepatocytes. .. Mouse tissue was homogenized in 100 μl Complete Lysis M‐buffer (Roche Applied Science, Indianapolis, IN, lifescience.roche.com ) over ice and centrifuged for 15 minutes at 4°C 10 rpm.

    Gene Expression:

    Article Title: Limited effects of preterm birth and the first enteral nutrition on cerebellum morphology and gene expression in piglets
    Article Snippet: Preamplified and exonuclease‐treated cDNA was diluted 1:10 in low EDTA TE‐buffer (VWR, Bie & Berntsen, Copenhagen, Denmark) before qPCR. .. Expression analysis was performed in two 96/96 Dynamic Array Integrated Fluidic Circuits (Fluidigm, South San Francisco, CA) using TaqMan Gene Expression Master Mix (Life Technologies, Carlsbad, CA), EvaGreen 20X (VWR), and gene‐specific primers as described previously (Skovgaard et al. ). ..

    Article Title: Lymphocyte soluble factors from pregnant cows modulate mRNA transcript abundances encoding for proteins associated with trophoblast growth and development.
    Article Snippet: All primers were designed and produced by Fluidigm (Fluidigm, San Francisco, CA). .. The single cell gene expression protocol was used with Fluidigm 96.96 Dynamic Array Integrated Fluidic Circuits (Fluidigm, San Francisco, CA) and included steps for reverse transcription, target amplification, and sample dilution prior to being loaded into the Fluidigm chip. .. Manufacturer instructions for priming and loading the 96.96 Fluidigm chip were followed, and the loaded chip was placed into the Biomark real-time PCR machine for qPCR (Fluidigm, San Francisco, CA).

    Sequencing:

    Article Title: Targeted massively parallel sequencing of a panel of putative breast cancer susceptibility genes in a large cohort of multiple-case breast and ovarian cancer families
    Article Snippet: .. Family sequencing and segregation analysis Segregation analysis for all putative pathogenic mutations was carried out by either Sanger sequencing using the standard sequencing procedure of BigDye Terminator V.3.1 Cycle Sequencing Kit in the PE Applied Biosystem (PE Applied Biosystem) or by Fluidigm 96.96 Dynamic Array integrated fluidic circuits on the BioMark HD System (Fluidigm Corporation) according to the manufacturer's instructions. ..

    Article Title: Targeted massively parallel sequencing of a panel of putative breast cancer susceptibility genes in a large cohort of multiple-case breast and ovarian cancer families
    Article Snippet: .. Segregation analysis for all putative pathogenic mutations was carried out by either Sanger sequencing using the standard sequencing procedure of BigDye Terminator V.3.1 Cycle Sequencing Kit in the PE Applied Biosystem (PE Applied Biosystem) or by Fluidigm 96.96 Dynamic Array integrated fluidic circuits on the BioMark HD System (Fluidigm Corporation) according to the manufacturer's instructions. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Ionizing radiations sustain glioblastoma cell dedifferentiation to a stem-like phenotype through survivin: possible involvement in radioresistance
    Article Snippet: Total RNA was isolated either from primary NS, FCS-differentiated cells or from cells at the end of the dedifferentiation protocol using RNeasy kit (Qiagen, Courtaboeuf, France) and then reverse-transcribed using iScript cDNA synthesis kit (Bio-Rad, Marnes la Coquette, France). .. Real-time qPCR reactions were carried out using the Fluidigm 96.96 dynamic array integrated fluidic circuits and the Biomark HD System (Fluidigm, Les Ulis, France) according Advanced Development Protocol no. 37 (Toulouse GeT Platform). β 2-Microglobulin was used as endogenous control in the ΔCt analysis. .. The different primers (Eurogentec, Angers, France) used in this study are described in Supplementary Table 1 .

    Article Title: FGFR1/FOXM1 pathway: a key regulator of glioblastoma stem cells radioresistance and a prognosis biomarker
    Article Snippet: Total RNAs were isolated either from Neurospheres or GBM-differentiated cells using RNeasy kit (Qiagen, Hilden, Germany) and then reverse-transcribed using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). .. Real-time qPCR reactions were carried out using Evagreen dye and ABI-Stepone+ Detection System (Applied Biosystems, Foster City, CA, USA) or the Fluidigm 96.96 dynamic array integrated fluidic circuits and the Biomark HD System according Advanced Development Protocol n°37 (Toulouse GeT Platform, France), β2-microglobulin (β2M) or Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as endogenous control in the ΔCt analysis. ..

    Control:

    Article Title: Ionizing radiations sustain glioblastoma cell dedifferentiation to a stem-like phenotype through survivin: possible involvement in radioresistance
    Article Snippet: Total RNA was isolated either from primary NS, FCS-differentiated cells or from cells at the end of the dedifferentiation protocol using RNeasy kit (Qiagen, Courtaboeuf, France) and then reverse-transcribed using iScript cDNA synthesis kit (Bio-Rad, Marnes la Coquette, France). .. Real-time qPCR reactions were carried out using the Fluidigm 96.96 dynamic array integrated fluidic circuits and the Biomark HD System (Fluidigm, Les Ulis, France) according Advanced Development Protocol no. 37 (Toulouse GeT Platform). β 2-Microglobulin was used as endogenous control in the ΔCt analysis. .. The different primers (Eurogentec, Angers, France) used in this study are described in Supplementary Table 1 .

    Article Title: FGFR1/FOXM1 pathway: a key regulator of glioblastoma stem cells radioresistance and a prognosis biomarker
    Article Snippet: Total RNAs were isolated either from Neurospheres or GBM-differentiated cells using RNeasy kit (Qiagen, Hilden, Germany) and then reverse-transcribed using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). .. Real-time qPCR reactions were carried out using Evagreen dye and ABI-Stepone+ Detection System (Applied Biosystems, Foster City, CA, USA) or the Fluidigm 96.96 dynamic array integrated fluidic circuits and the Biomark HD System according Advanced Development Protocol n°37 (Toulouse GeT Platform, France), β2-microglobulin (β2M) or Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as endogenous control in the ΔCt analysis. ..

    Reverse Transcription:

    Article Title: Lymphocyte soluble factors from pregnant cows modulate mRNA transcript abundances encoding for proteins associated with trophoblast growth and development.
    Article Snippet: All primers were designed and produced by Fluidigm (Fluidigm, San Francisco, CA). .. The single cell gene expression protocol was used with Fluidigm 96.96 Dynamic Array Integrated Fluidic Circuits (Fluidigm, San Francisco, CA) and included steps for reverse transcription, target amplification, and sample dilution prior to being loaded into the Fluidigm chip. .. Manufacturer instructions for priming and loading the 96.96 Fluidigm chip were followed, and the loaded chip was placed into the Biomark real-time PCR machine for qPCR (Fluidigm, San Francisco, CA).

    Amplification:

    Article Title: Lymphocyte soluble factors from pregnant cows modulate mRNA transcript abundances encoding for proteins associated with trophoblast growth and development.
    Article Snippet: All primers were designed and produced by Fluidigm (Fluidigm, San Francisco, CA). .. The single cell gene expression protocol was used with Fluidigm 96.96 Dynamic Array Integrated Fluidic Circuits (Fluidigm, San Francisco, CA) and included steps for reverse transcription, target amplification, and sample dilution prior to being loaded into the Fluidigm chip. .. Manufacturer instructions for priming and loading the 96.96 Fluidigm chip were followed, and the loaded chip was placed into the Biomark real-time PCR machine for qPCR (Fluidigm, San Francisco, CA).



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